Protection against influenza virus infection by intranasal vaccine with surf clam microparticles (SMP) as an adjuvant
Identifieur interne : 004198 ( Main/Exploration ); précédent : 004197; suivant : 004199Protection against influenza virus infection by intranasal vaccine with surf clam microparticles (SMP) as an adjuvant
Auteurs : Takeshi Ichinohe [Japon] ; Izumi Watanabe [Japon] ; Eriko Tao [Japon] ; Satoshi Ito [Japon] ; Akira Kawaguchi [Japon] ; Shin-Ichi Tamura [Japon] ; Hidehiro Takahashi [Japon] ; Hirofumi Sawa [Japon] ; Masami Moriyama [Japon] ; Joe Chiba [Japon] ; Katsuhiro Komase [Japon] ; Yujiro Suzuki [Japon] ; Takeshi Kurata [Japon] ; Tetsutaro Sata [Japon] ; Hideki Hasegawa [Japon]Source :
- Journal of Medical Virology [ 0146-6615 ] ; 2006-07.
English descriptors
- Teeft :
- Adjuvant, Aizawa, Assay, Body weight, Bronchoalveolar wash, Chitin microparticles, Cholera, Cholera toxin, Clam, Complete protection, Control mice, Effective mucosal adjuvant, Elisa, Escherichia coli enterotoxin, Hasegawa, Heterologous, Heterologous viruses, Holotoxin, Ichinohe, Immunization, Immunization protocol, Immunol, Immunol tamura, Infection, Infectious diseases, Initial body weight, Intracerebral, Intragastric, Intragastric administration, Intranasal, Intranasal administration, Intranasal immunization, Intranasal vaccination, Intranasal vaccine, Intranasally, Kurata, Lethal lung infection, Lethal pneumonia, Lethal virus challenge, Lung wash, Mdck, Mdck cells, Mice vaccinated, Microparticles, Mouse, Mucosal, Nasal, Nasal cavity, Nasal mucosa, Nasal vaccination, Nasal vaccine, Nasal wash, National institute, Neutralizing, Neutralizing activity, Other adjuvants, Plaque assay, Present study, Safe adjuvant, Subunit, Surf, Surf clam microparticles, Survival rates, Suzuki, Tamura, Time course, Titer, Toxin, Trace amount, Vaccinated, Vaccination, Vaccine, Vaccine tamura, Various adjuvants, Virol, Virus, Virus challenge, Virus infection, Virus titer, Virus titers, Watanabe.
Abstract
A safe and effective adjuvant is necessary to enhance mucosal immune responses for the development of an inactivated intranasal influenza vaccine. The present study demonstrated the effectiveness of surf clam microparticles (SMP) derived from natural surf clams as an adjuvant for an intranasal influenza vaccine. The adjuvant effect of SMP was examined when co‐administered intranasally with inactivated A/PR8 (H1N1) influenza virus hemagglutinin vaccine in BALB/c mice. Administration of the vaccine with SMP induced a high anti‐PR8 haemagglutinin (HA)‐specific immunoglobulin A (IgA) response in the nasal wash and immunoglobulin G (IgG) response in the serum, resulting in protection against both nasal‐restricted infection and lethal lung infection by A/PR8 virus. In addition, administration of SMP with A/Yamagata (H1N1), A/Beijing (H1N1), or A/Guizhou (H3N2) vaccine conferred complete protection against A/PR8 virus challenge in the nasal infection model, suggesting that SMP adjuvanted vaccine can confer cross‐protection against variant influenza viruses. The use of SMP is suggested as a new safe and effective mucosal adjuvant for nasal vaccination against influenza virus infection. J. Med. Virol. 78:954–963, 2006. © 2006 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jmv.20647
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001845
- to stream Istex, to step Curation: 001845
- to stream Istex, to step Checkpoint: 001624
- to stream Main, to step Merge: 004414
- to stream Main, to step Curation: 004198
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Protection against influenza virus infection by intranasal vaccine with surf clam microparticles (SMP) as an adjuvant</title><author><name sortKey="Ichinohe, Takeshi" sort="Ichinohe, Takeshi" uniqKey="Ichinohe T" first="Takeshi" last="Ichinohe">Takeshi Ichinohe</name></author><author><name sortKey="Watanabe, Izumi" sort="Watanabe, Izumi" uniqKey="Watanabe I" first="Izumi" last="Watanabe">Izumi Watanabe</name></author><author><name sortKey="Tao, Eriko" sort="Tao, Eriko" uniqKey="Tao E" first="Eriko" last="Tao">Eriko Tao</name></author><author><name sortKey="Ito, Satoshi" sort="Ito, Satoshi" uniqKey="Ito S" first="Satoshi" last="Ito">Satoshi Ito</name></author><author><name sortKey="Kawaguchi, Akira" sort="Kawaguchi, Akira" uniqKey="Kawaguchi A" first="Akira" last="Kawaguchi">Akira Kawaguchi</name></author><author><name sortKey="Tamura, Shin Chi" sort="Tamura, Shin Chi" uniqKey="Tamura S" first="Shin-Ichi" last="Tamura">Shin-Ichi Tamura</name></author><author><name sortKey="Takahashi, Hidehiro" sort="Takahashi, Hidehiro" uniqKey="Takahashi H" first="Hidehiro" last="Takahashi">Hidehiro Takahashi</name></author><author><name sortKey="Sawa, Hirofumi" sort="Sawa, Hirofumi" uniqKey="Sawa H" first="Hirofumi" last="Sawa">Hirofumi Sawa</name></author><author><name sortKey="Moriyama, Masami" sort="Moriyama, Masami" uniqKey="Moriyama M" first="Masami" last="Moriyama">Masami Moriyama</name></author><author><name sortKey="Chiba, Joe" sort="Chiba, Joe" uniqKey="Chiba J" first="Joe" last="Chiba">Joe Chiba</name></author><author><name sortKey="Komase, Katsuhiro" sort="Komase, Katsuhiro" uniqKey="Komase K" first="Katsuhiro" last="Komase">Katsuhiro Komase</name></author><author><name sortKey="Suzuki, Yujiro" sort="Suzuki, Yujiro" uniqKey="Suzuki Y" first="Yujiro" last="Suzuki">Yujiro Suzuki</name></author><author><name sortKey="Kurata, Takeshi" sort="Kurata, Takeshi" uniqKey="Kurata T" first="Takeshi" last="Kurata">Takeshi Kurata</name></author><author><name sortKey="Sata, Tetsutaro" sort="Sata, Tetsutaro" uniqKey="Sata T" first="Tetsutaro" last="Sata">Tetsutaro Sata</name></author><author><name sortKey="Hasegawa, Hideki" sort="Hasegawa, Hideki" uniqKey="Hasegawa H" first="Hideki" last="Hasegawa">Hideki Hasegawa</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:FA21B2348E0EFDA47D5E7B31E3EC2AD1855B1478</idno><date when="2006" year="2006">2006</date><idno type="doi">10.1002/jmv.20647</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-9VSCQ5CZ-X/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001845</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001845</idno><idno type="wicri:Area/Istex/Curation">001845</idno><idno type="wicri:Area/Istex/Checkpoint">001624</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001624</idno><idno type="wicri:doubleKey">0146-6615:2006:Ichinohe T:protection:against:influenza</idno><idno type="wicri:Area/Main/Merge">004414</idno><idno type="wicri:Area/Main/Curation">004198</idno><idno type="wicri:Area/Main/Exploration">004198</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Protection against influenza virus infection by intranasal vaccine with surf clam microparticles (SMP) as an adjuvant</title><author><name sortKey="Ichinohe, Takeshi" sort="Ichinohe, Takeshi" uniqKey="Ichinohe T" first="Takeshi" last="Ichinohe">Takeshi Ichinohe</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Pathology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Biological Science and Technology, Tokyo University of Science, Chiba</wicri:regionArea><wicri:noRegion>Chiba</wicri:noRegion></affiliation></author><author><name sortKey="Watanabe, Izumi" sort="Watanabe, Izumi" uniqKey="Watanabe I" first="Izumi" last="Watanabe">Izumi Watanabe</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Pathology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Biological Science and Technology, Tokyo University of Science, Chiba</wicri:regionArea><wicri:noRegion>Chiba</wicri:noRegion></affiliation></author><author><name sortKey="Tao, Eriko" sort="Tao, Eriko" uniqKey="Tao E" first="Eriko" last="Tao">Eriko Tao</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Pathology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Ito, Satoshi" sort="Ito, Satoshi" uniqKey="Ito S" first="Satoshi" last="Ito">Satoshi Ito</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Pathology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Biological Science and Technology, Tokyo University of Science, Chiba</wicri:regionArea><wicri:noRegion>Chiba</wicri:noRegion></affiliation></author><author><name sortKey="Kawaguchi, Akira" sort="Kawaguchi, Akira" uniqKey="Kawaguchi A" first="Akira" last="Kawaguchi">Akira Kawaguchi</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Pathology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Biological Science and Technology, Tokyo University of Science, Chiba</wicri:regionArea><wicri:noRegion>Chiba</wicri:noRegion></affiliation></author><author><name sortKey="Tamura, Shin Chi" sort="Tamura, Shin Chi" uniqKey="Tamura S" first="Shin-Ichi" last="Tamura">Shin-Ichi Tamura</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Pathology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Takahashi, Hidehiro" sort="Takahashi, Hidehiro" uniqKey="Takahashi H" first="Hidehiro" last="Takahashi">Hidehiro Takahashi</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Pathology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Sawa, Hirofumi" sort="Sawa, Hirofumi" uniqKey="Sawa H" first="Hirofumi" last="Sawa">Hirofumi Sawa</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Molecular Pathobiology and 21st Century COE Program for Zoonosis Control, Hokkaido University Research Center for Zoonosis Control, Sapporo</wicri:regionArea><wicri:noRegion>Sapporo</wicri:noRegion></affiliation></author><author><name sortKey="Moriyama, Masami" sort="Moriyama, Masami" uniqKey="Moriyama M" first="Masami" last="Moriyama">Masami Moriyama</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Microbiology and Immunology, School of Medicine, Keio University, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Chiba, Joe" sort="Chiba, Joe" uniqKey="Chiba J" first="Joe" last="Chiba">Joe Chiba</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Biological Science and Technology, Tokyo University of Science, Chiba</wicri:regionArea><wicri:noRegion>Chiba</wicri:noRegion></affiliation></author><author><name sortKey="Komase, Katsuhiro" sort="Komase, Katsuhiro" uniqKey="Komase K" first="Katsuhiro" last="Komase">Katsuhiro Komase</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Research Center for Biologicals, Kitasato Institute, Kitamoto, Saitama</wicri:regionArea><wicri:noRegion>Saitama</wicri:noRegion></affiliation></author><author><name sortKey="Suzuki, Yujiro" sort="Suzuki, Yujiro" uniqKey="Suzuki Y" first="Yujiro" last="Suzuki">Yujiro Suzuki</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Research Center for Biologicals, Kitasato Institute, Kitamoto, Saitama</wicri:regionArea><wicri:noRegion>Saitama</wicri:noRegion></affiliation></author><author><name sortKey="Kurata, Takeshi" sort="Kurata, Takeshi" uniqKey="Kurata T" first="Takeshi" last="Kurata">Takeshi Kurata</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Pathology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Sata, Tetsutaro" sort="Sata, Tetsutaro" uniqKey="Sata T" first="Tetsutaro" last="Sata">Tetsutaro Sata</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Pathology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Hasegawa, Hideki" sort="Hasegawa, Hideki" uniqKey="Hasegawa H" first="Hideki" last="Hasegawa">Hideki Hasegawa</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Pathology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Japon</country></affiliation><affiliation wicri:level="1"><country xml:lang="fr" wicri:curation="lc">Japon</country><wicri:regionArea>Correspondence address: Department of Pathology, National Institute of Infectious Diseases, 4‐7‐1 Gakuen, Musashimurayama‐shi, Tokyo 208‐0011</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Medical Virology</title><title level="j" type="alt">JOURNAL OF MEDICAL VIROLOGY</title><idno type="ISSN">0146-6615</idno><idno type="eISSN">1096-9071</idno><imprint><biblScope unit="vol">78</biblScope><biblScope unit="issue">7</biblScope><biblScope unit="page" from="954">954</biblScope><biblScope unit="page" to="963">963</biblScope><biblScope unit="page-count">10</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2006-07">2006-07</date></imprint><idno type="ISSN">0146-6615</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0146-6615</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Adjuvant</term><term>Aizawa</term><term>Assay</term><term>Body weight</term><term>Bronchoalveolar wash</term><term>Chitin microparticles</term><term>Cholera</term><term>Cholera toxin</term><term>Clam</term><term>Complete protection</term><term>Control mice</term><term>Effective mucosal adjuvant</term><term>Elisa</term><term>Escherichia coli enterotoxin</term><term>Hasegawa</term><term>Heterologous</term><term>Heterologous viruses</term><term>Holotoxin</term><term>Ichinohe</term><term>Immunization</term><term>Immunization protocol</term><term>Immunol</term><term>Immunol tamura</term><term>Infection</term><term>Infectious diseases</term><term>Initial body weight</term><term>Intracerebral</term><term>Intragastric</term><term>Intragastric administration</term><term>Intranasal</term><term>Intranasal administration</term><term>Intranasal immunization</term><term>Intranasal vaccination</term><term>Intranasal vaccine</term><term>Intranasally</term><term>Kurata</term><term>Lethal lung infection</term><term>Lethal pneumonia</term><term>Lethal virus challenge</term><term>Lung wash</term><term>Mdck</term><term>Mdck cells</term><term>Mice vaccinated</term><term>Microparticles</term><term>Mouse</term><term>Mucosal</term><term>Nasal</term><term>Nasal cavity</term><term>Nasal mucosa</term><term>Nasal vaccination</term><term>Nasal vaccine</term><term>Nasal wash</term><term>National institute</term><term>Neutralizing</term><term>Neutralizing activity</term><term>Other adjuvants</term><term>Plaque assay</term><term>Present study</term><term>Safe adjuvant</term><term>Subunit</term><term>Surf</term><term>Surf clam microparticles</term><term>Survival rates</term><term>Suzuki</term><term>Tamura</term><term>Time course</term><term>Titer</term><term>Toxin</term><term>Trace amount</term><term>Vaccinated</term><term>Vaccination</term><term>Vaccine</term><term>Vaccine tamura</term><term>Various adjuvants</term><term>Virol</term><term>Virus</term><term>Virus challenge</term><term>Virus infection</term><term>Virus titer</term><term>Virus titers</term><term>Watanabe</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A safe and effective adjuvant is necessary to enhance mucosal immune responses for the development of an inactivated intranasal influenza vaccine. The present study demonstrated the effectiveness of surf clam microparticles (SMP) derived from natural surf clams as an adjuvant for an intranasal influenza vaccine. The adjuvant effect of SMP was examined when co‐administered intranasally with inactivated A/PR8 (H1N1) influenza virus hemagglutinin vaccine in BALB/c mice. Administration of the vaccine with SMP induced a high anti‐PR8 haemagglutinin (HA)‐specific immunoglobulin A (IgA) response in the nasal wash and immunoglobulin G (IgG) response in the serum, resulting in protection against both nasal‐restricted infection and lethal lung infection by A/PR8 virus. In addition, administration of SMP with A/Yamagata (H1N1), A/Beijing (H1N1), or A/Guizhou (H3N2) vaccine conferred complete protection against A/PR8 virus challenge in the nasal infection model, suggesting that SMP adjuvanted vaccine can confer cross‐protection against variant influenza viruses. The use of SMP is suggested as a new safe and effective mucosal adjuvant for nasal vaccination against influenza virus infection. J. Med. Virol. 78:954–963, 2006. © 2006 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>Japon</li></country><region><li>Région de Kantō</li></region><settlement><li>Tokyo</li></settlement></list><tree><country name="Japon"><region name="Région de Kantō"><name sortKey="Ichinohe, Takeshi" sort="Ichinohe, Takeshi" uniqKey="Ichinohe T" first="Takeshi" last="Ichinohe">Takeshi Ichinohe</name></region><name sortKey="Chiba, Joe" sort="Chiba, Joe" uniqKey="Chiba J" first="Joe" last="Chiba">Joe Chiba</name><name sortKey="Hasegawa, Hideki" sort="Hasegawa, Hideki" uniqKey="Hasegawa H" first="Hideki" last="Hasegawa">Hideki Hasegawa</name><name sortKey="Hasegawa, Hideki" sort="Hasegawa, Hideki" uniqKey="Hasegawa H" first="Hideki" last="Hasegawa">Hideki Hasegawa</name><name sortKey="Hasegawa, Hideki" sort="Hasegawa, Hideki" uniqKey="Hasegawa H" first="Hideki" last="Hasegawa">Hideki Hasegawa</name><name sortKey="Ichinohe, Takeshi" sort="Ichinohe, Takeshi" uniqKey="Ichinohe T" first="Takeshi" last="Ichinohe">Takeshi Ichinohe</name><name sortKey="Ito, Satoshi" sort="Ito, Satoshi" uniqKey="Ito S" first="Satoshi" last="Ito">Satoshi Ito</name><name sortKey="Ito, Satoshi" sort="Ito, Satoshi" uniqKey="Ito S" first="Satoshi" last="Ito">Satoshi Ito</name><name sortKey="Kawaguchi, Akira" sort="Kawaguchi, Akira" uniqKey="Kawaguchi A" first="Akira" last="Kawaguchi">Akira Kawaguchi</name><name sortKey="Kawaguchi, Akira" sort="Kawaguchi, Akira" uniqKey="Kawaguchi A" first="Akira" last="Kawaguchi">Akira Kawaguchi</name><name sortKey="Komase, Katsuhiro" sort="Komase, Katsuhiro" uniqKey="Komase K" first="Katsuhiro" last="Komase">Katsuhiro Komase</name><name sortKey="Kurata, Takeshi" sort="Kurata, Takeshi" uniqKey="Kurata T" first="Takeshi" last="Kurata">Takeshi Kurata</name><name sortKey="Moriyama, Masami" sort="Moriyama, Masami" uniqKey="Moriyama M" first="Masami" last="Moriyama">Masami Moriyama</name><name sortKey="Sata, Tetsutaro" sort="Sata, Tetsutaro" uniqKey="Sata T" first="Tetsutaro" last="Sata">Tetsutaro Sata</name><name sortKey="Sawa, Hirofumi" sort="Sawa, Hirofumi" uniqKey="Sawa H" first="Hirofumi" last="Sawa">Hirofumi Sawa</name><name sortKey="Suzuki, Yujiro" sort="Suzuki, Yujiro" uniqKey="Suzuki Y" first="Yujiro" last="Suzuki">Yujiro Suzuki</name><name sortKey="Takahashi, Hidehiro" sort="Takahashi, Hidehiro" uniqKey="Takahashi H" first="Hidehiro" last="Takahashi">Hidehiro Takahashi</name><name sortKey="Tamura, Shin Chi" sort="Tamura, Shin Chi" uniqKey="Tamura S" first="Shin-Ichi" last="Tamura">Shin-Ichi Tamura</name><name sortKey="Tao, Eriko" sort="Tao, Eriko" uniqKey="Tao E" first="Eriko" last="Tao">Eriko Tao</name><name sortKey="Watanabe, Izumi" sort="Watanabe, Izumi" uniqKey="Watanabe I" first="Izumi" last="Watanabe">Izumi Watanabe</name><name sortKey="Watanabe, Izumi" sort="Watanabe, Izumi" uniqKey="Watanabe I" first="Izumi" last="Watanabe">Izumi Watanabe</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004198 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004198 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:FA21B2348E0EFDA47D5E7B31E3EC2AD1855B1478
|texte= Protection against influenza virus infection by intranasal vaccine with surf clam microparticles (SMP) as an adjuvant
}}
| This area was generated with Dilib version V0.6.33. |  |